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40-man roster
| 63 | Nick Anderson | P |
| 44 | Dairon Blanco | LF |
| 62 | Jonathan Bowlan | P |
| 59 | Jake Brentz | P |
| 64 | Steven Cruz | P |
| 34 | Freddy Fermin | C |
| 26 | Adam Frazier | 2B |
| 11 | Maikel Garcia | 3B |
| 27 | Tyler Gentry | RF |
| 2 | Garrett Hampson | CF |
| 43 | Carlos Hernandez | P |
| 28 | Kyle Isbel | CF |
| 36 | Will Klein | P |
| 12 | Nick Loftin | 2B |
| 67 | Seth Lugo | P |
| 24 | Jordan Lyles | P |
| 41 | Daniel Lynch IV | P |
| 48 | Alec Marsh | P |
| 19 | Michael Massey | 2B |
| 66 | James McArthur | P |
| 58 | John McMillon | P |
| 1 | MJ Melendez | LF |
| 14 | Austin Nola | C |
| 9 | Vinnie Pasquantino | 1B |
| 13 | Salvador Perez | C |
| 32 | Nick Pratto | 1B |
| 55 | Cole Ragans | P |
| 16 | Hunter Renfroe | RF |
| 65 | Matt Sauer | P |
| 46 | John Schreiber | P |
| Colin Selby | P | |
| 51 | Brady Singer | P |
| 31 | Will Smith | P |
| 35 | Chris Stratton | P |
| 17 | Nelson Velazquez | LF |
| 69 | Anthony Veneziano | P |
| 52 | Michael Wacha | P |
| 6 | Drew Waters | CF |
| 7 | Bobby Witt Jr. | SS |
| 61 | Angel Zerpa | P |